Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: IL-4 and IL-13 guide early thymic progenitors to mature towards dendritic cells

doi: 10.4049/jimmunol.1701186

Figure Lengend Snippet: (A) Thymic cells from HR+/+ IL-13Rα1-GFP reporter mice were depleted of Lin+ cells and the Lin-CD4-CD8- cells were stained with antibodies to CD25, CD44, c-Kit, and intracellular pSTAT6(Y641), pSTAT1(Y701), pSTAT1(S727), pSTAT3 (S727) and pSTAT3 (Y705). The CD25-CD44+c-Kit+GFP+ (HR+ETPs) and CD25-CD44+c-Kit+GFP- (HR-/PETPs) were then analysed ex vivo for phosphorylation of STAT6, STAT1, and STAT3 at the indicated aa residues in comparison to isotype control for the corresponding anti-STAT antibody. The histograms show a representative experiment and the bar graphs show the mean ± SD MFI results compiled from 3 independent experiments. **p<0.01 as determined by two-tailed, unpaired Student’s t-test. (B, C) Thymic cells from HR−/− mice were depleted of Lin+ cells and the Lin-CD4-CD8- cells were stained with antibodies to CD25, CD44 and c-Kit, sorted as CD25-CD44+c-Kit+HR−/− ETPs, and transduced with HR-RV to drive HR expression. The cells were stained with anti-Thy1.1 and anti-IL-13Rα1 (marker for HR expression) and sorted as IL-13Rα1+Thy1.1+ cells. HR-RV transduced ETPs were cultured in the presence of 10ng/ml IL-4 alone (IL-4) or with STAT1 (IL-4 +STAT1 I) or STAT6 (IL-4 + STAT6 I) inhibitor. Similar culture conditions were used with 20 ng/ml IL-13 alone (IL-13) or with STAT1 (IL-13 + STAT1 I) or STAT6 (IL-13 + STAT6 I) inhibitor. Culture without cytokine or inhibitor (NIL) was included for control purposes. (B) STAT1 and ( C) STAT6 phosphorylation was analyzed after 1 hour incubation by flow cytometry. The histograms show representative experiments for STAT1 and STAT6 phosphorylation while the bar graphs show the mean ± SD MFI results compiled from three independent experiments. *p<0.05, **p<0.01 as determined by one-way ANOVA.

Article Snippet: Anti-CD3 (145–2C11), anti-CD4 (RM4–5), anti-CD8 (53–6.7), anti-CD25 (7D4), anti-CD44 (IM7), anti-CD45 (30-F11), anti-Pax5 (1H9), anti-CD45.1 (A20), anti-CD11b (M1/70), anti-CD11c (HL3), anti-CD117 (2B8), anti-IFNγR1 (XMG1.2), anti-pSTAT6 Y641 (J71–773.58.11), anti-pSTAT3 S727 (49/p-Stat3), anti-pSTAT3 Y705 (4/P-STAT3), anti-Zbtb46 (U4–1374), anti-SIRPα (P84), and anti-CD90.1 (OX-7), were purchased from BD biosciences (San Jose, CA).

Techniques: Staining, Ex Vivo, Two Tailed Test, Transduction, Expressing, Marker, Cell Culture, Incubation, Flow Cytometry

Journal: iScience

Article Title: Sleep deprivation-induced sympathetic activation promotes pro-tumoral macrophage phenotype via the ADRB2/KLF4 pathway to facilitate NSCLC metastasis

doi: 10.1016/j.isci.2025.112321

Figure Lengend Snippet:

Article Snippet: anti- pSTAT6 , ImmunoWay , Cat# YP0256.

Techniques: Recombinant, Transfection, Enzyme-linked Immunosorbent Assay, Phagocytosis Assay, Sequencing, Software

Journal: Cell reports

Article Title: Mass Cytometry Reveals Global Immune Remodeling with Multi-lineage Hypersensitivity to Type I Interferon in Down Syndrome

doi: 10.1016/j.celrep.2019.10.038

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-human phospho-STAT6 (Tyr641) (clone 18) , Fluidigm , Cat#3168012A; RRID:AB_2811103.

Techniques: Recombinant, Mass Cytometry, Software